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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Drug-free in vitro activation combined with 3D-bioprinted adipose-derived stem cells restores ovarian function of rats with premature ovarian insufficiency

Fig. 4

Proliferation and apoptosis of granulosa cells (GCs) after graft transplantation. Representative immunohistochemical images of Ki67 (A) and caspase-3 (B) in six different groups. Scale bar: 50 µm and 200 µm. C Representative immunofluorescence staining of PCNA in six different groups. Scale bar: 500um and 100 µm. D Representative pictures of TUNEL staining from the four treatment groups. Green staining represents apoptotic GCs. Scale bar: 50um. The mean optical density (MOD) of ki67-positive (E) and caspase-3-positive areas (F) were analyzed by Image-Pro Plus. (* versus the normal group, # versus the POI group, & comparison between two groups; *, #, & P < 0.05; **, ##, && P < 0.01; ***, ###, &&& P < 0.001)

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