Fig. 6

Promoted angiogenesis by regulating PI3K/AKT pathway and secreting proangiogenic factors. A Representative VEGF, p-PI3K, PI3K, p-AKT and AKT bands of PI3K/AKT pathway using GAPDH as the internal reference. B Quantitative analysis of protein expression showed that the expression level of p-PI3K, p-AKT and VEGF was increased in fragment-cell group and 3D-bioprinted engineering ovary group, while the level of PI3K and AKT did not show significant difference in different groups. C The mRNA levels of VEGF, FGF-2, and angiogenin were upregulated in the 3D-bioprinted engineering ovary group. (* versus the normal group, # versus the POI group, & comparison between two groups; *, #, & P < 0.05; **, ##, && P < 0.01; ***, ###, &&& P < 0.001)