Table 2 Reagents details
From: Generation of an mESC model with a human hemophilia B nonsense mutation via CRISPR/Cas9 technology
Antibody | Dilution | Company Cat # and RRID | |
|---|---|---|---|
Antibodies used for immunocytochemistry | |||
Primary antibodies | Rabbit anti-NANOG | 1:500 | Novus Biologicals Cat #NB100-58842 RRID: AB_877697 |
Primary antibodies | Mouse anti-OCT3/4 | 1:200 | Santa Cruz Biotechnology Cat#sc-5279 RRID: AB_628051 |
Primary antibodies | Rabbit anti-Factor IX | 1:500 | Invitrogen Cat# PA5-22304 RRID: AB_11153706 |
Secondary antibodies | Goat anti- Mouse IgG (H + L) Alexa Fluor 488 | 1:500 | Thermo Fisher Scientific Cat# A-11001 RRID: AB_2534069 |
Secondary antibodies | Goat anti- Rabbit IgG (H + L) Alexa Fluor 488 | 1:500 | Thermo Fisher Scientific Cat# A-11008 RRID: AB_143165 |
Antibodies used for Western blot | |||
Primary antibodies | Rabbit anti-Factor-IX | 1:1000 | Abcam Cat#ab255824 |
Secondary antibodies | Goat anti-Rabbit IgG H&L (HRP) | 1:1000 | Abcam Cat#ab6721 RRID: AB_955447 |