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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Exosomal miR-17-5p from adipose-derived mesenchymal stem cells inhibits abdominal aortic aneurysm by suppressing TXNIP-NLRP3 inflammasome

Fig. 1

Isolation and characterization of ADSCs and ADSC-exos. A ADSC-specific surface markers such as CD29, CD31, CD34, CD45, CD90, and CD105 measured by flow cytometry. B P3-ADSCs in vitro showed typical spindle fibroblast-like morphology. Scale bar: 100 μm. C Positive Oil-Red-O staining of ADSCs was assessed after adipogenic differentiation induction for 2 weeks. Scale bar: 100 μm. D Positive Alcian Blue staining of ADSCs assessed after osteogenic differentiation induction for 3 weeks. Scale bar: 100 μm. E Western blot of exosomal biomarkers. F, G The ADSC-derived exosomes showed a circular disk shape under TEM (F) with the average particle size at 100 nm according to NTA analysis (G). ADSC, Adipose-derived mesenchymal stem cell; P3, passage 3

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Stem Cell Research & Therapy

ISSN: 1757-6512