| Current study (tECM < 100 kDa) | Ref. [26] | Ref. [27] | Ref. [28] | Ref. [29] |
---|---|---|---|---|---|
Species | Bovines | Equine | Equine | Human | Canidae |
Sample source | Achilles tendon | SDFT | SDFT | Patellar tendon | LDET |
Sample preparation | 3Â M Urea | 4Â M GuHCL, 65Â mM DTT | 0.1% Rapigest | 0.1% Rapigest in 50Â mM ammonium bicarbonate | 4Â M GuHCL, 65Â mM DTT |
Protein digestion | In-gel trypsin digestion | In-solution trypsin digestion | In-solution trypsin digestion | In-solution trypsin digestion | In-solution trypsin digestion |
Extracellular matrix (ECM) protein | Total: 19 | Total: 36 | Total: 34 | Total: 63 | Total: 85 |
Collagen | 2 | – | 15 | 13 | 11 |
Proteoglycan | 8 | – | 7 | 8 | 13 |
Glycoprotein | 5 | – | 8 | 24 | 30 |
Main identified Collagen | COL1A1, COL1A2 | COL1A1, COL1A2, COL3A1, COL4A1 | COL1A1, COL1A2, COL12A1 | COL1A1, COL1A2, COL3A1, COL12A1 | COL1A1, COL1A2, COL6A1, COL6A2, COL12A1 |
Main identified non-collagen ECM protein | KERA, DCN, BGN, FMOD, PRELP, OGN | DCN, FMOD, COMP, THBS4, PRELP | FN, THBS4, FMOD, COMP, CILP1, THBS1 | FMOD, FN1, PRELP, COMP | DCN, BGN, LUM, TNC |
Identified protein-enriched GO functional annotation | Extracellular matrix; Collagen binding | Intermediate filament; Extracellular matrix | Organization of collagen fibrils and filaments | – | ECM organization; Wound healing; Collagen fibril organization |
Identified protein-enriched pathway | ECM-receptor interaction; Focal adhesion | ECM-receptor interaction; Focal adhesion | – | – | – |