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Table 3 Comparison of tendon ECM proteomic study methodology and results

From: Tenogenic induction of human adipose-derived stem cells by soluble tendon extracellular matrix: composition and transcriptomic analyses

 

Current study

(tECM < 100 kDa)

Ref. [26]

Ref. [27]

Ref. [28]

Ref. [29]

Species

Bovines

Equine

Equine

Human

Canidae

Sample source

Achilles tendon

SDFT

SDFT

Patellar tendon

LDET

Sample preparation

3 M Urea

4 M GuHCL, 65 mM DTT

0.1% Rapigest

0.1% Rapigest in 50 mM ammonium bicarbonate

4 M GuHCL, 65 mM DTT

Protein digestion

In-gel trypsin digestion

In-solution trypsin digestion

In-solution trypsin digestion

In-solution trypsin digestion

In-solution trypsin digestion

Extracellular matrix (ECM) protein

Total: 19

Total: 36

Total: 34

Total: 63

Total: 85

Collagen

2

–

15

13

11

Proteoglycan

8

–

7

8

13

Glycoprotein

5

–

8

24

30

Main identified Collagen

COL1A1, COL1A2

COL1A1, COL1A2, COL3A1, COL4A1

COL1A1, COL1A2, COL12A1

COL1A1, COL1A2, COL3A1, COL12A1

COL1A1, COL1A2, COL6A1, COL6A2, COL12A1

Main identified non-collagen ECM protein

KERA, DCN, BGN, FMOD, PRELP, OGN

DCN, FMOD, COMP, THBS4, PRELP

FN, THBS4, FMOD, COMP, CILP1, THBS1

FMOD, FN1, PRELP, COMP

DCN, BGN, LUM, TNC

Identified protein-enriched GO functional annotation

Extracellular matrix; Collagen binding

Intermediate filament; Extracellular matrix

Organization of collagen fibrils and filaments

–

ECM organization; Wound healing; Collagen fibril organization

Identified protein-enriched pathway

ECM-receptor interaction; Focal adhesion

ECM-receptor interaction; Focal adhesion

–

–

–