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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Podocytes derived from human induced pluripotent stem cells: characterization, comparison, and modeling of diabetic kidney disease

Fig. 2

Validation of each stage of podocyte differentiation. Immunostaining of each stage of differentiation showing A pluripotency marker octamer-binding transcription factor 4 (OCT-4) at day 1; B primitive streak marker MIXL1 at day 2; C intermediate mesoderm marker PAX8 at day 5; and D nephron progenitor markers SIX2 and CITED1 at day 7. Corresponding flow cytometry analysis of the markers was plotted on the y-axis for E OCT-4, F MIXL1, G PAX8, and H SIX2 versus forward scatter (FSC) on the x-axis (n = 2). Scale bar is 100 μm

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