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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Podocytes derived from human induced pluripotent stem cells: characterization, comparison, and modeling of diabetic kidney disease

Fig. 4

Comparison of the protocol with existing protocols. A Schematic of the podocyte differentiation protocols compared including key media components. B(i) Comparison of the podocyte markers MAFB and PODXL expression using flow cytometry analysis (n = 3). (ii) Quantification of (Bi). C Immunostaining of iPSC-podocytes with markers PODXL, NPHS1 in the compared protocols (n = 3). Scale bar 100 μm. D Western blot analysis of the podocyte markers PODXL, SYNPO, NPHS1, and WT1 as well as loading control β-actin in the compared protocols (n = 3)

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