Fig. 6

Modeling diabetic kidney disease by high glucose treatment of iPSC-derived podocytes. A Brightfield image of cisplatin- or high glucose-treated podocytes showing damage to the junctions between cells (arrows). Scale bar represents 50 μm. B(i) Reorganization of F-actin (phalloidin-red) along the cell periphery after the treatment with either Cisplatin (5 µM) or high glucose (100 mM). Scale bar is 100 μm. (ii) Higher magnification images showing out-of-order and intertwined actin fibers in treated groups compared to control groups podocytes with paralleled fasciculate models (n = 2). Scale bar is 30 μm. C Viability analysis by MTT assay showing reduction in podocyte viability after treatment with glucose. D Cytotoxicity analysis using LDH assay indicating increased toxicity within the podocytes treated with glucose. *Indicates p < 0.05