Cell type | Target | Origin | Isotype | Company | Catalog # | Dilution |
---|---|---|---|---|---|---|
Primary antibodies | ||||||
Undifferentiated | OCT-4 | Rat | IgG2b | R&D systems | MAB1759SP | 1:200 |
Primitive streak | MIXL1 | Rabbit | IgG | Proteintech | 22772-1-AP | 1:200 |
Intermediate mesoderm | PAX8 | Rabbit | IgG | Proteintech | 10336-1-AP | 1:200 |
Nephron progenitors | CITED1 | Mouse | IgG2a | Fisher scientific | 89-335-107 | 1:200 |
SIX2 | Rabbit | IgG | Proteintech | 11562-1-AP | 1:200 | |
Podocytes | PODXL | Rabbit | IgG | Proteintech | 18150-1-AP | 1:200 |
NEPHRIN (NPHS1) | Sheep | IgG | R&D systems | AF4269 | 1:200 | |
SYNPO | Rabbit | IgG | Abcam | ab224491 | 1:200 | |
Podocytes (mouse-specific) | NEPHRIN (NPHS1) | Mouse | IgG | R&D systems | AF3159-SP | 1:200 |
Podocytes (human specific) | MAFB | Rabbit | IgG | Abcam | ab223744 | 1:200 |
Visualization of | Binds to | Stain | Channel | Company | Catalog # | Dilution |
---|---|---|---|---|---|---|
Stains | ||||||
Cytoskeleton | F-actin | Phalloidin | 594 | Molecular Probes | A12381 | 1:100 |
Nuclei | DNA | DAPI | blue | Millipore Sigma | D9542 | 1:1000 |
Species | Fluorophore | Target | Company | Catalog # | Dilution |
---|---|---|---|---|---|
Secondary antibodies | |||||
Goat | Alexa 488 | Anti-mouse IgGa | Life technologies | A-21131 | 1:200 |
Goat | Alexa 488 | Anti-rat IgG | Life technologies | A-11006 | 1:400 |
Goat | Alexa 594 | Anti-rabbit IgG | Life technologies | A-11037 | 1:400 |
Donkey | Alexa 594 | Anti-sheep IgG | Life technologies | A-11016 | 1:400 |