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Table 1 Primary antibodies, secondary antibodies, and stains for flow cytometry, immunostaining, and immunoblotting experiments

From: Podocytes derived from human induced pluripotent stem cells: characterization, comparison, and modeling of diabetic kidney disease

Cell type

Target

Origin

Isotype

Company

Catalog #

Dilution

Primary antibodies

Undifferentiated

OCT-4

Rat

IgG2b

R&D systems

MAB1759SP

1:200

Primitive streak

MIXL1

Rabbit

IgG

Proteintech

22772-1-AP

1:200

Intermediate mesoderm

PAX8

Rabbit

IgG

Proteintech

10336-1-AP

1:200

Nephron progenitors

CITED1

Mouse

IgG2a

Fisher scientific

89-335-107

1:200

 

SIX2

Rabbit

IgG

Proteintech

11562-1-AP

1:200

Podocytes

PODXL

Rabbit

IgG

Proteintech

18150-1-AP

1:200

 

NEPHRIN (NPHS1)

Sheep

IgG

R&D systems

AF4269

1:200

 

SYNPO

Rabbit

IgG

Abcam

ab224491

1:200

Podocytes (mouse-specific)

NEPHRIN (NPHS1)

Mouse

IgG

R&D systems

AF3159-SP

1:200

Podocytes (human specific)

MAFB

Rabbit

IgG

Abcam

ab223744

1:200

Visualization of

Binds to

Stain

Channel

Company

Catalog #

Dilution

Stains

      

Cytoskeleton

F-actin

Phalloidin

594

Molecular Probes

A12381

1:100

Nuclei

DNA

DAPI

blue

Millipore Sigma

D9542

1:1000

Species

Fluorophore

Target

Company

Catalog #

Dilution

Secondary antibodies

Goat

Alexa 488

Anti-mouse IgGa

Life technologies

A-21131

1:200

Goat

Alexa 488

Anti-rat IgG

Life technologies

A-11006

1:400

Goat

Alexa 594

Anti-rabbit IgG

Life technologies

A-11037

1:400

Donkey

Alexa 594

Anti-sheep IgG

Life technologies

A-11016

1:400