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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Endogenous Follistatin-like 1 guarantees the immunomodulatory properties of mesenchymal stem cells during liver fibrotic therapy

Fig. 3

FSTL1low MSCs lose their immunosuppressive capacity on inflammatory macrophages in fibrotic mouse model. A and B: Infiltrating inflammatory macrophages and cytokines in liver tissue were evaluated 1 weeks after MSCs transplantation. Pro-inflammatory macrophages (iNOS +) and anti-inflammatory macrophages (CD206 +) were analysed using IHC staining (A). Cytokines in liver tissues related to inflammatory macrophages were evaluated by qRT-PCR analyses (B). C–E: Non-parenchymal cells in livers were isolated and analysed at 1 week post MSC transplantation for subsequent analyses. Total number of macrophages (F4/80 + CD11b +) in the fibrotic liver (C and D, n = 4). (E) Mean fluorescence intensity (MFI) of inflammatory surface markers on macrophages (CD11c, CD206, CD86) after MSC treatment (n = 4). **, P < 0.01; *, P < 0.05. Bars, 100 µm. All experiments were performed in triplicate. Error bars indicate the mean ± SEM

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