Fig. 4
FSTL1low MSCs lose their immunosuppressive function on macrophages in vitro. A-B: MSC transfected with siFSTL1treated with or without FSTL1 (100 ng/ml) were co-cultured with Raw264.7 cells. The proportions of CD206-positive macrophages were determined after 24 h co-culture with MSCs (A). The mean percentages of CD206-positive cells in total macrophages were summarised (B). C, D: MSCs transfected with siFSTL1 treated with or without FSTL1 (100 ng/ml) were co-cultured with Raw264.7 cells after 24 h IFN-γand LPS treatment. The proportions of CD86-positive cells were subsequently determined (C). Mean percentages of CD86-positive cells in total macrophages were summarised after 24 h co-culture with MSCs (D). E Expression of differential markers in macrophages evaluated by qPCR under different conditions after 12 h co-culture with MSCs. **, P < 0.01; *, P < 0.05. All experiments were performed in triplicate. Error bars indicate the mean ± SEM