Fig. 2
Nondiabetic plasma-treated SVFs suppress M1 but promote IL-10 mRNA expression in the adipose tissue of Leprdb/db mice. SVFs were harvested from adipose tissue of Leprdb/db mice and treated with plasma from Lepr+/+ or Leprdb/db mice. Lepr+/+ plasma- or Leprdb/db plasma-treated SVFs were injected into the inguinal WAT of Leprdb/db mice. Lepr+/+ and Leprdb/db mice were also treated with PBS as controls. One week later, the inguinal WAT was harvested from Lepr+/+ mice treated with PBS and Leprdb/db mice treated with PBS, Lepr+/+ plasma-treated SVFs, or Lepr.db/db plasma-treated SVFs. SVFs were then harvested from WAT followed by Q-PCR analysis for mRNA expression of TNFα, IL-1β, IL-33, IL-6, IL-10, and DPP4. N = 5/group. *p < 0.05, **p < 0.01, ***p < 0.001