Fig. 4

Nondiabetic plasma-treated SVFs increase Foxp3 expression and CD4⁺ Tregs in the adipose tissue of Lepr^db/db mice. (A) Lepr^db/db SVFs were treated with PBS, Lepr^+/+ plasma, or Lepr^db/db plasma, and Lepr^+/+ SVFs were treated with PBS. 3.5 h after treatment, ATMs and NATMs were isolated from SVFs using the microbeads followed by Q-PCR analysis for Foxp3 mRNA expression. (B) Lepr^db/db SVFs were treated with Lepr^+/+ or Lepr^db/db plasma and then injected into the inguinal WAT of Lepr^db/db mice. Lepr^+/+ and Lepr^db/db mice were also treated with PBS as controls. One week later, ATMs and NATMs were isolated from adipose tissue using the microbeads and then subjected to Q-PCR analysis for Foxp3 mRNA expression. (C and D) Flow cytometry analysis was used to assess the frequency and number of CD4.⁺ Tregs in the adipose tissue. N = 5/group. *p < 0.05