Fig. 1

The gating strategy for the study of immune cell subsets by flow cytometry. Initially, CD45⁺ cells were identified. Then, CD19⁺ cells were sorted out. Subsequently CD3⁺, CD4⁺ and CD8⁺ cells were sorted out from CD45⁺ and CD19⁻ cells. CD16⁺ cells were then sorted out from CD45⁺, CD19⁻, CD⁻, CD3⁻ cells. Activated lymphocytes (CD69⁺) were identified within the CD45⁺ cell-population. HLA-ABC⁺ and HLA-DR⁺ cells were also identified among the CD45⁺ cell-population