Fig. 9

The results of flow cytometry for immune cell subsets from samples obtained from a representative example of a diabetic humanized mouse that underwent transplantation: A Blood sample: CD19⁺ cells accounted for 35% and CD69⁺ for 0.7% of the CD45⁺ cells. CD4⁺ cells accounted for 57% and CD8⁺ for 34% of the CD3⁺ population. The proportion of CD45⁺, CD19⁻, CD3⁻ and CD16⁺ cells was 6.6%. The proportion of cells expressing HLA-ABC and of HLA-DR was 98% and 42%, respectively. B Splenocytes: CD19⁺ cells accounted for 57% and CD69⁺ cells for 5.5% of CD45⁺ cells. The CD3⁺ cells and CD4⁺ cells accounted for 51% and CD8⁺ cells for 41% of the CD3⁺ cells. The proportion of CD45⁺, CD19⁻, CD3⁻ and CD16⁺ cells was 1.8%. The proportion of cells expressing HLA-ABC and of HLA-DR were 87% and 49%, respectively. C Bone marrow: CD19⁺ cells accounted for 51% and CD69⁺ cells for 11.4% of the CD45⁺ cells. CD4⁺ cells accounted for 73% and CD8 + cells for 17% of the CD3⁺ cell population. The proportion of CD45⁺, CD19⁻, CD3⁻ and CD16⁺ cells was 3.8%. The proportion of cells expressing HLA-ABC and of HLA-DR was 82% and 74%, respectively