Fig. 3From: Ficoll density gradient sedimentation isolation of pelage hair follicle mesenchymal stem cells from adult mouse back skin: a novel method for hair follicle mesenchymal stem cells isolationMSC Characteristics of Pelage DPCs and Whisker DPCs. Flow cytometry tested for mouse MSC specific markers and three-linage differentiation was performed. Pelage DPCs highly expressed CD29 (94.02%), CD90 (89.47%) and Sca-1 (80.56%), but hardly expressed CD45 (1.97%) and CD44 (2.6%) (A). Pelage DPCs showed adipogenic (B, Oil Red O staining), osteogenic (C, Alizarin Red staining) and chondrogenic (D, Alcian Blue staining) differentiation ability. As the control, whisker DPCs showed similar MSC markers expression (E) and also similar ability of adipogenic (F), osteogenic (G) and chondrogenic (H) differentiationBack to article page