Fig. 2
From: Role of TRPC6 in periodontal tissue reconstruction mediated by appropriate stress
Mechanical force upregulated TRPC6 expression in PDLSCs. A The expression of TRPC6 as determined by western blotting and quantitative analysis of TRPC6 expression in hPDLSCs are shown. B The immunohistochemical images indicate that the expression of TRPC6 increased in molars subjected to orthodontic force. The black arrow indicates the direction of orthodontic force. C, crown; R, root; P, periodontal ligament; A, alveolar bone; Black scale bar, 1 mm; White scale bar, 500 μm; Blue scale bar, 200 μm. C Schematic diagrams. D Semiquantitative analysis of TRPC6-positive cells in immunohistochemical images. E The results of real-time PCR showed that the expression of TRPC6 was significantly increased when hPDLSCs were exposed to a 3 μm confined microenvironment for 24 h. All the results are expressed as the mean ± standard deviation of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001; ns, P > 0.05