Fig. 3
From: Role of TRPC6 in periodontal tissue reconstruction mediated by appropriate stress
HPDLSCs in the 5 μm and 8 μm confined microenvironments. A In the 5 μm confined microenvironment, the actin bundles in the cytoskeleton disappeared, and the cell body became round and disordered. In the 8 μm confined microenvironment, the changes in the cytoskeleton were not obvious. Under treatment with the TRPC6 inhibitor SKF96365, the ability of cells to resist compressive stimulation decreased; thus, the cell morphology exhibited a relaxed state. Scale bar, 20 μm. B The cell migration trajectory decreased in the confined microenvironment. Environmental factors played a key regulatory role, and the role of TRPC6 was also significant in the 8 μm confined microenvironment. The results are expressed as the mean ± standard deviation of one independent experiment; N ≥ 3. *P < 0.05; ***P < 0.001; ns, P > 0.05