Fig. 1From: miR-126-3p containing exosomes derived from human umbilical cord mesenchymal stem cells promote angiogenesis and attenuate ovarian granulosa cell apoptosis in a preclinical rat model of premature ovarian failureIdentification of hucMSCs and hucMSCs-exosomes. A. Primary hucMSCs exhibited a typical fibroblastic morphology 14 days after culture (40). B. The cells were positive for CD29, CD44, CD166, CD90 and CD105 and negative for CD34 and CD45. C. hucMSCs differentiated into osteocytes by alizarin red staining (100). D. hucMSCs differentiated into adipo-lineage cells by oil red o staining (200). E. Expression of green fluorescent protein in miR-126-3p–transfected hucMSCs (200). F. The expression of miR-126-3p in hucMSCs-exosomes was detected by PCR G. Morphology of isolated exosomes using TEM. H. The mean diameters of the isolated exosomes. I. Expression levels of TSG101, HSP70, CD63, and calnexin in hucMSCs-exosomes were detected by western blot. J. The expression of miR-126-3p in hucMSCs-exosomes was detected by PCR. *P < 0.05 for all figuresBack to article page