Fig. 11

The expression levels of SIRT6, NF-κB, and BDNF in the ovaries were detected using immunohistochemical staining. a–c A brown-yellow color indicates positive expression of the target protein. The white arrow represents SIRT6 expression, the red arrow represents NF-κB expression, and the green arrow represents BDNF expression. Scale bar: 200 μm. d–f The statistical charts of the three kinds of protein expression in the four groups. SIRT6, sirtuin 6; NF-κB, nuclear factor kappa B; and BDNF, brain-derived neurotrophic factor (ns = not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.001)