Fig. 3

Knockdown of lnc-BIHAA1 suppresses the activation of HSC-T6 induced by TGF-β1. A The graph shows the density of each chromosome that total mapped reads compared with the genome. B Distribution of differentially expressed lncRNA on chromosomes. C The heat map of differentially expressed lncRNA. GO (D) and KEGG (E) analysis shows the possible function and pathways affected by the differentially expressed lncRNAs. F The differentially expressed lncRNAs’ response to the TGF-β1 induction was verified by qRT-PCR. G qRT-PCR shows the expression lncRNA response to BMSC treatment in the hepatic fibrosis rats. H qRT-PCR verifies the knockdown of lnc-BIHAA1 by siRNAs. I CCK-8 assay showing the effect of lnc-BIHAA1 siRNA on the activity of TGF-β1 inducted HSC-T6 cells. qPCR (J), WB (K), and IHC (L) assay showing the effect of lnc-BIHAA1 siRNA on the expression of fibrosis markers in TGF-β1-induced HSC-T6 cells. Magnification, 200×. M FISH assay showing the cellular location of lnc-BIHAA1 in HSC-T6 cells