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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Human umbilical cord mesenchymal stem cell-derived exosomal miR-335-5p attenuates the inflammation and tubular epithelial–myofibroblast transdifferentiation of renal tubular epithelial cells by reducing ADAM19 protein levels

Fig. 6

ADAM19 expression is inhibited by human umbilical cord mesenchymal stem cell (hucMSC)-exosome treatment in TGF-β1-treated HK-2 cells. A Venn image showing the overlapping targeted genes among StarBase3.0, miRWalk, and GSE20247 dataset analyses. B The expression of ADAM19, ASTN2, and RCOR1 was measured by RT-qPCR in TGF-β1-treated HK-2 cells (n = 3). C The binding site of the ADAM19 3′-UTR and miR-335-5p is shown, and their binding was verified by luciferase analysis. D The protein level of ADAM19 in TGF-β1-treated HK-2 cells, measured by western blotting, was inhibited by hucMSC-exosome treatment or miR-335-5p mimic transfection (n = 3). (*P < 0.05, vs NC mimic group). All statistical analyses were performed by student's t test

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