Fig. 4
From: Hyaluronan supports the limbal stem cell phenotype during ex vivo culture
Effects of HA on TKE2 cells cultured on differently coated dishes or in the presence of HA as a supplement to the media. TKE2 cells were cultured on differently coated dishes (A) or on uncoated dishes with HA, HA + anti-CD44 or 4MU + Hyalase supplemented to the media (B). The number of ∆Np63 positive cells and the total number of cells (DAPI) were quantified using QuPath [88] and the percentage of ∆Np63+ cells calculated (C). *Represents p ≤ 0.05, scale bar represents 50 μm