Fig. 5

Effects of HA on the differentiation of TKE2 cells cultured on differently coated dishes or in the presence of HA as a supplement to the media. TKE2 cells were cultured on differently coated dishes (A) or on uncoated dishes with HA of different molecular weights, HA + anti-CD44 or 4MU + Hyalase supplemented to the media (B) under differentiating conditions. The number of ∆Np63 positive cells and the total number of cells (DAPI) were quantified using Qupath [88] and the percentage of ∆Np63⁺ cells calculated for TKE cells maintained on differently coated dishes (C) and with HA, HA + anti-CD44 and 4MU + Hyalase supplemented to the media (D). *Represents p ≤ 0.05, scale bar represents 50 μm