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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Knockout of integrin β1 in induced pluripotent stem cells accelerates skin-wound healing by promoting cell migration in extracellular matrix

Fig. 3

Effects of Itgb1-knockout on iPSCs function and survival in the absence of Matrigel. a Representative fluorescence imaging (left) and quantification (right) of PKH26-labeled iPSCs adhered to plates without Matrigel (n = 5). b Representative fluorescence imaging (left) and quantification (right) of PKH26-labeled iPSCs migration across the chamber membrane in the absence of Matrigel (n = 3). c iPSC apoptosis in the absence of Matrigel measured by Annexin V/FITC staining (n = 3). d iPSC proliferation in the absence of Matrigel measured by CCK-8 assay (n = 6). p < 0.05, **p < 0.01, ***p < 0.001. OD, optical density; PI, propidium iodide

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