Fig. 5

Effects of Itgb1-knockout on Matrigel-embedded iPSC-mediated wound healing and iPSC survival in skin wound tissues. a Representative macroscopic images of cutaneous wounds treated with Matrigel- or agarose-embedded iPSCs on days 0, 2, 4, 6, 8, 10, and 12 post-treatment (left). Quantification of wound healing expressed as the percentage of healing relative to initial wound size at day 0 (right) (n = 10). b Representative photomicrographs of CD31 IHC staining of wound tissues on days 6 and 12 post-treatment (left). CD31 signal densities were determined using ImageJ software (right) (n = 5). c Quantification of Cd34 and Cd31 mRNA levels in wound tissues (n = 10). d Representative blood perfusion images of cutaneous wounds treated with iPSCs or an equal volume of PBS on days 0, 2, 4, 6, 8, 10, and 12 post-treatment (left). Quantification of blood perfusion in wound tissues (right) (n = 10). e Representative agarose gel electrophoresis images of Tet-On and 18S DNA (left). Quantification of Tet-on expression normalized against 18S expression in wound tissues (right) (n = 10). *p < 0.05, **p < 0.01, ***p < 0.0001