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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Propofol enhances stem-like properties of glioma via GABAAR‐dependent Src modulation of ZDHHC5-EZH2 palmitoylation mechanism

Fig. 4

Propofol upregulates ZDHHC5 transcripts by increasing the Src and p53 interaction. A The mRNA levels of ZDHHC5 in U118-derived GSCs treated with propofol in different concentrations were analyzed by RT-PCR (n = 5 in each group). β-actin was used as the loading control. B ChIP was performed on U118-derived GSCs with the indicated p53 and Src antibodies. PCR analysis was performed on the endogenous promoters of ZDHHC5 and cdk1 genes; cdk1 was used as a positive control; and the primers for unrelated target sites of ZDHHC5 (ZDHHC5 NT) were used as a negative control. C An immunoprecipitation assay was performed on U118-derived GSCs with the indicated p53 and Src antibodies. D Luciferase reporter assays with the wild-type ZDHHC5 reporter vector, p53 and Src, alone or in combination, in U118-derived GSCs. The luciferase values were relative to the Renilla luciferase activity (n = 7 in each group). E Detection of glioma spheres formed for U118-derived GSCs treated with or without propofol, Src siRNA or Src inhibitor, PP2, and cultured for a second passage. Quantification of the glioma sphere-formation capacity (n = 5 in each group). Western blotting for p-Src and Src in GSCs treated with or without propofol, Src siRNA or Src inhibitor, PP2 (n = 5 in each group). GAPDH was used as a loading control

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