Fig. 5From: Downregulation of DNA methyltransferase-3a ameliorates the osteogenic differentiation ability of adipose-derived stem cells in diabetic osteoporosis via Wnt/β-catenin signaling pathwayDnmt siRNAs increase the osteogenic potential of DOP-ASCs. A: Alizarin Red-S staining showing that the formation of mineralized nodules in Dnmt3a shRNA was increased compared with that in Negative Control after Dnmt siRNA treatment of DOP-ASCs (osteoinduction for 21 days). B–E: mRNA and protein levels of Wnt/β-Catenin signaling pathway markers and osteogenesis-related molecules were upregulated after Dnmt siRNA transfection into DOP-ASCs (osteoinduction for 4 days). Data represent the mean ± SD of at least three independent experiments, *P < 0.05, **P < 0.01Back to article page