Fig. 8

BCP was successfully implanted into the skull defect of mice. BCP seeded with transfected DOP-ASCs were implanted into critically sized calvarial defects in DOP mouse models. A, B: mRNA and protein levels of Dnmt3a were successfully knocked down by Dnmt3a shRNA (osteoinduction for 3 days). C: SEM and fluorescence microscopy showing that DOP-ASCs grew adherently on the surface and pores of BCP. D: The DOP mouse model with critically sized calvarial defects was successfully established and BCP seeded with transfected DOP-ASCs were implanted into the defect area