Stem Cell Research & Therapy

Table 1 Primer sequences for RT-PCR

From: Downregulation of DNA methyltransferase-3a ameliorates the osteogenic differentiation ability of adipose-derived stem cells in diabetic osteoporosis via Wnt/β-catenin signaling pathway

Genes		Sequence (5′ → 3′)
Gapdh	Forward	GGTGAAGGTCGGTGTGAACG
Gapdh	Reverse	CTCGCTCCTGGAAGATGGTG
Runx2	Forward	CCGAACTGGTCCGCACCGAC
Runx2	Reverse	CTTGAAGGCCACGGGCAGGG
Opn	Forward	GGATTCTGTGGACTCGGATG
Opn	Reverse	CGACTGTAGGGACGATTGGA
Dnmt1	Forward	CCGAACTGGTCCGCACCGAC
Dnmt1	Reverse	CTTGAAGGCCACGGGCAGGG
Dnmt3a	Forward	GAGGGAACTGAGACCCCAC
Dnmt3a	Reverse	CTGGAAGGTGAGTCTTGGCA
Dnmt3b	Forward	AGCGGGTATGAGGAGTGCAT
Dnmt3b	Reverse	GGGAGCATCCTTCGTGTCTG
β-Catenin	Forward	AAGTTCTTGGCTATTACGACA
β-Catenin	Reverse	ACAGCACCTTCAGCACTCT
Lef1	Forward	ACAGATCACCCCACCTTCTTG
Lef1	Reverse	TGATGGGAAAACCTGGACAT

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ISSN: 1757-6512

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