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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Topical administration of the secretome derived from human amniotic epithelial cells ameliorates psoriasis-like skin lesions in mice

Fig. 6

AEC-SC inhibited the inflammation stimulated by TNFα in HaCaT karatinocytes and IL-1ra mediated the function of AEC-SC. a The relative mRNA levels of IL-1β, IL-6 and CCL20 in HaCaT cells in each group as indicated. Data was collected from at least 3 separate experiments and the data are expressed as the mean ± SEM.*P < 0.05, **P < 0.01 and ***P < 0.001. b Western blot analysis of the protein levels of NF-κb p-P65 and MAPK p-P38 in the cell lysates of HaCaT cells. HaCaT cells were pretreated with AEC-SC or AEC-SC + IL-1ra Ab or IL-1ra for 4 h, then stimulated with TNFα for 15 min. c Qualification of relative protein levels of p-P65 and p-P38 in the cell lysates

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