Fig. 5

Irisin-mediated up-regulation of miR-125b-5p targeting SIPA1L2 to promote chondrogenic differentiation of hMSCs. A Human MSCs was seeded in 24-wells plates via high-density micromass culture, and chondrogenic differentiation was induced in chondrogenic medium with or without irisin (100 ng/ml) treatment for 7 days. Differently miRNA expressions in control and irisin-treated group were shown. B Relative expressions of the indicated miRNAs in cartilage tissues detected by qPCR. C The mRNA expressions (Left panel) of COL2A1, ACAN, and SOX9, and the protein expressions (Right panel) of SIPA1L2, COL2A1, ACAN, and SOX9 in the control, NC, and miR-125b-5p mimics transduction groups. D The mRNA expressions (Left panel) of COL2A1, ACAN, and SOX9, and the protein expressions (Right panel) of SIPA1L2, COL2A1, ACAN, and SOX9 in the control, NC, and miR-125b-5p inhibitor transduction groups under irisin treatment. E, F The expressions of SIPA1L2, COL2A1, and SOX9 were detected by immunofluorescence combined with DAPI staining for the nuclei in different groups. (Magnification: × 400, scale bar: 50 μm). G, H Safranin O and Alcian Blue staining were performed in cartilage tissue paraffin sections with different treatments as shown. (Magnification: × 200, scale bar: 100 μm). The concentration of irisin used in this figure was 100 ng/ml. *P < 0.05, **P < 0.01 compared with the control group or NC group