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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Circular RNA BIRC6 depletion promotes osteogenic differentiation of periodontal ligament stem cells via the miR-543/PTEN/PI3K/AKT/mTOR signaling pathway in the inflammatory microenvironment

Fig. 1

The effects of inflammatory microenvironment on the proliferation and osteogenic differentiation of PDLSCs. A MTT assay of PDLSCs in the normal condition and inflammatory environment. B Representative images of the EdU assay in PDLSCs with the indicated treatments. Scale bar: 50 μm. C The flow cytometry revealed the cell cycle distribution of PDLSCs with the indicated treatments. D ALP staining of PDLSCs following a 10-day osteogenic differentiation induction in the normal and inflammatory microenvironment. E ARS staining of PDLSCs following a three-week osteogenic differentiation induction with the indicated treatments. F, G qRT-PCR and immunoblot of osteogenic differentiation markers in PDLSCs with indicated treatments. H Immunofluorescence staining of RUNX2 and COL1A1 in PDLSCs in the normal and inflammatory conditions. Scale bar: 20 μm. Data are presented as the mean ± SD (ns: not significant, **P < 0.01, ***P < 0.001)

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