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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Circular RNA BIRC6 depletion promotes osteogenic differentiation of periodontal ligament stem cells via the miR-543/PTEN/PI3K/AKT/mTOR signaling pathway in the inflammatory microenvironment

Fig. 2

CircBIRC6 depletion promoted the osteogenic differentiation of PDLSCs under the inflammatory microenvironment. A CircBIRC6 level was significantly higher in PDLSCs exposed to inflammatory stimulus. B The expression level of circBIRC6 was markedly higher in periodontitis tissues compared to normal tissues. C Establishment of circBIRC6-stable knockdown PDLSCs, and the relative levels of circBIRC6 and linear BIRC6 were detected with qRT-PCR. D, E Flow cytometry and EdU assays showed that downregulation of circBIRC6 had little effect on the proliferative capacity of PDLSCs under the inflammatory conditions. Scale bar: 50 μm. F The ALP staining intensity was higher in the circBIRC6 knockdown groups compared to the control group. G ARS assay of the mineralized nodules formation capacity of PDLSCs with the indicated modifications. H–J qRT-PCR, immunoblot and immunofluorescence staining of osteogenic differentiation markers in PDLSCs with the indicated modifications. Scale bar: 20 μm. Data are presented as the mean ± SD (ns: not significant, *P < 0.05, **P < 0.01, ***P < 0.001)

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Stem Cell Research & Therapy

ISSN: 1757-6512