Fig. 4

CircBIRC6 acted as an efficient miR-543 sponge in PDLSCs. A qRT-PCR was performed to evaluate the relative expression levels of potential target miRNAs pulled down by the circBIRC6 probe or oligo probe. B RIP assay was conducted with anti-AGO2 or anti-IgG in PDLSCs after transfection with the miR-543 mimic or NC mimic, and qRT-PCR was used to detect the relative enrichment of circBIRC6. C Relative luciferase activities in PDLSCs co-transfected with circBIRC6 wt or circBIRC6 mut and the miR-543 mimic, miR-543 inhibitor or respective negative controls. D The correlation between circBIRC6 and miR-543 in periodontitis tissues was evaluated. Data are presented as the mean ± SD (ns: not significant, **P < 0.01, ***P < 0.001)