Fig. 5

MiR-543 promoted the osteogenic differentiation of PDLSCs under the inflammatory microenvironment. A The expression of miR-543 in PDLSCs was analyzed by qRT-PCR after transfection with miR-543 inhibitor and NC inhibitor. B–F The osteogenic differentiation capability of PDLSCs transfected with miR-543 inhibitor in the inflammatory conditions was evaluated by ALP staining, ARS staining, qRT-PCR, WB and immunofluorescence staining. G The miR-543 level was detected with qRT-PCR after transfection with miR-543 mimic and NC mimic. H–L The effects of miR-543 mimic on the osteogenic differentiation of PDLSCs under the inflammatory microenvironment were evaluated by ALP staining, ARS staining, qRT-PCR, WB and immunofluorescence staining. Data are presented as the mean ± SD (*P < 0.05, **P < 0.01, ***P < 0.001). Scale bar: 20 μm (F and L)