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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Insufficient S-adenosylhomocysteine hydrolase compromises the beneficial effect of diabetic BMSCs on diabetic cardiomyopathy

Fig. 1

The distinctive effect on diabetic cardiomyopathy of BMSCs harvested from healthy and diabetic rats. BMSCs were infused 4 times, and cardiac function was measured using echocardiography (a) in control and DCM rats. The LVEF (b) and E′/A′ ratio (c) were used to assess the cardiac systolic and diastolic function, respectively (n = 7–8). Heart samples were harvested and fixed with paraformaldehyde (n = 6). The HE staining (upper) and Masson’s trichrome staining (lower) were conducted to assess myocardial morphology and cardiac fibrosis (d). The relative area of myocardial fibrosis (e) was estimated (scale bar = 20 μm). qPCR analysis showed the increased expression of cardiac stress-related genes, including Nppa, Nppb, and Myh7 (f) in rats with DCM (n = 4), suggesting adverse ventricular remodeling. The MDA levels (g, nmol/mg) and DHE staining (h, i) of cardiac tissue were measured to reflect the increased oxidative stress in rats with DCM (scale bar = 40 μm, n = 7). *P < 0.05 versus normal group, #P < 0.05 versus DCM group, and P < 0.05 versus DM-BSMC group

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