Fig. 4

The effect of SAHH inhibition in the normal BMSCs on endothelial cells. Western blots showed that plasmid containing SAHH shRNA and ADA significantly reduced the expression of SAHH in normal BMSCs (a, b, n = 5). According to the CCK-8 assay (c), SAHH inhibition reduced the cellular variability of BMSCs (n = 5). *P < 0.05 versus normal group. After SAHH inhibition, nondiabetic BMSCs could not promote endothelial proliferation (d, e) and tube formation (f, g, n = 5). *P < 0.05 versus normal group, #P < 0.05 versus BMSCs group. ROS generation (h, i) in endothelial cells under high glucose and fatty acids was measured using DHE staining after treatment with or without medium derived from normal BMSCs (n = 5). *P < 0.05 versus normal group, #P < 0.05 versus HG/PA group, and P < 0.05 versus HG/PA +BMSCs group.