Fig. 3

MSC-EVs promote photoreceptor survival in rd10 mice at P28. A Images of eye cups with retinas stained with DAPI from three animal groups, with squared regions at 600 µm away from the center of the optic disk (OD) enlarged in B. ONL layer where photoreceptor somas are located (*) became thinner in rd10 mice, while MSC-EVs increased its thickness. C, D Images of immunofluorescence staining with an antibody against recoverin (green, C) or PSD95 (red, D) and DAPI (blue) of retinal slices in three animal groups. Arrowheads in D point to the photoreceptors presynaptic terminals that are labeled by anti-PSD95. Pink arrows point to blood vessels. E Quantification of the ONL thickness at 200 µm, 400 µm, 600 µm, 1000 µm and 2000 µm away from the OD at P28. F Quantification of the mean gray intensity in the three animal groups of recoverin (F) and PSD95 (G) in three animal groups. OD, optic disk; ONL, outer nuclear layer; INL, inner nuclear layer; and GCL, ganglion cell layer. Data are shown as mean ± SEM. * P < 0.05, **, P < 0.01. Student’s t test and one-way ANOVA were applied