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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Mesenchymal stem cell-derived extracellular vesicles protect retina in a mouse model of retinitis pigmentosa by anti-inflammation through miR-146a-Nr4a3 axis

Fig. 4

MSC-EVs inhibit the reactive gliosis and immunoproliferation in the rd10 retina. A Retinal sections stained with DAPI (blue) and for Iba1 (green) in WT, NS-treated rd10 and MSC-EVs-treated rd10. In the rd10 retina, microglial cells presented as active state and distributed throughout the whole retina. MSC-EVs decreased its expression. B Retinal sections stained for GFAP (green). In the rd10 retina, GFAP was strongly expressed in Müller cells and extended from GCL to retinal OS; MSC-EVs decreased its expression. C-E Retinal sections stained for F4/80 (green, C), CD68 (pink, D) or CD206 (red, E). F, G Quantification of the Iba1-positive fluorescence intensity (F) and GFAP-positive fluorescence intensity (G). H-J Quantification of the number of F4/80 (M0 macrophage) (H)-, CD68 (M1 macrophage) (I)- and CD206 (M2 macrophage) (J)-positive cells. K, L Quantification of the ratio of M1/M0 number (K) and M2/M0 number (L). The MSC-EVs treatment reduced the fluorescence intensity of Iba1, the GFAP neurite intensity and the number of M0 and M1 macrophages in the rd10 retinas. White arrow indicated the cell somas of positive fluorescence. Data are shown as mean ± SEM. * P < 0.05, **, P < 0.01. One-way ANOVA

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