Fig. 8

MiR-146a directly regulates the expression of Nr4a3. A, B The expression of miR-146a-5p (A) and Nr4a3 (B) after MSC-EVs treatment for 24 h in 661 W cells. C, D The expression of miR-146a-5p (C) and Nr4a3 (D) after overexpressing miR-146a-5p by using the mimic or blocking it using inhibitor in 661 W cells. E, F The expression of miR-146a-5p(E) and Nr4a3 (F) after MSC-EVs treatment in BV2 cells. G, H The expression of miR-146a-5p (G) and Nr4a3 (H) after using the mimic or inhibitor in BV2 cells. The expression of Nr4a3 followed the changes of miR-146a-5p. I The luciferase activity of miR-146a-5p and Nr4a3 or with two forms mutated at the predicted binding sites of Nr4a3. Values were normalized to that of Nr4a3-negative control. J Overexpressing miR-146a in BV2 cells downregulated expression of the LPS-induced pro-inflammatory factors IL-1β, TNF-α and IL-6. Data are shown as mean ± SEM. One-way ANOVA and Student’s t test were applied for statistics. All experiments were repeated for 3 times