Table 1 Real-time RT-PCR Primers
Gene | Forward primer | Reverse primer |
|---|---|---|
Human | ||
Gapdh | TCAAGAAGGTGGTGAAGCAGG | TCAAAGGTGGAGGAGTGGGT |
Ido1 | GCCCTTCAAGTGTTTCACCAA | CCAGCCAGACAAATATATGCGA |
Rat | ||
Gapdh | TGTGAACGGATTTGGCCGTA | ATGAAGGGGTCGTTGATGGC |
Ido1 | AGCACTGGAGAAGGCACTG | CGTGGAAAAAGGTGTCTGG |
Nppb | CAATCCACGATGCAGAAGCTG | GGCGCTGTCTTGAGACCTAA |
Nppa | TGGGTCTTGTTAGGGCTCAAACCT | TGAAACTCAAGGGACACCCATCGT |
Mmp2 | ACAACAGCTGTACCACCGAG | GGACATAGCAGTCTCTGGGC |
Mmp9 | GATCCCCAGAGCGTTACTCG | GTTGTGGAAACTCACACGCC |
Acta2 | CGCCATCAGGAACCTCGAGAAG | ATCATCACCAGCAAAGCCCG |
Col1a1 | GACTGTCCCAACCCCCAAAA | CTTGGGTCCCTCGACTCCTA |
Col3a1 | TCCCCTGGAATCTGTGAATC | TGAGTCGAATTGGGGAGAAT |
Timp1 | CAGCAAAAGGCCTTCGTAAA | TGGCTGAACAGGGAAACACT |
Tbet | AACCAGTATCCTGTTCCCAGC | TGTCGCCACTGGAAGGATAG |
Gata3 | AGCCAGGAGAGCAGGGACG | CTGTTAATATTGTGAAGCTTGTAGTAGAG |
Rorγt | CCCATCTATGAGGGTTACGC | TTTAATGTCACGCACGATTTC |
Foxp3 | ACCGTATCTCCTGAGTTCCAT | GTCCAGCTTGACCACAGTTTAT |
Ifng | TACACGCCGCGTCTTGGT | GAGTGTGCCTTGGCAGTAACAG |
Tnfa | AACTCGAGTGACAAGCCCGTAG | GTACCACCAGTTGGTTGTCTTTG |
Il2 | GATCCAGCGATACGAGAACTT | CTCTGCATCTGGACCGTCA |
Il4 | AGCGGATCTCGGAAACTCAAT | CTGGCAGTCTGACATC |
Il5 | GAGGGGGCACTGTGGAAATA | ACTCATCACGCCAAGGAACT |
Il6 | GCCTATTGAAAATCTGCTCTGG | GGAAGTTGGGGTAGGAAGGA |
Il10 | GAAGATCTCAATAGCGTCA | AATCTCTCAATCCTCGTC |
Il12rβ2 | TTGCATCGCTATCATCGTGG | CCTCTTTTGAAGCAATAGGG |
Il13 | CCTGGAATCCCTGACCAACA | GCCATAGCGGAAAAGTTGCT |
Il17 | GGTACTCATCCCTCAAAGTTCA | CTCTTCAGGACCAGGATCTCT |
Il33 | GTGCAGGAAAGGAAGACTCG | TGGCCTCACCATAAGAAAGG |
Ccr5 | CACCCTGTTTCGCTGTAGGAATG | GCAGTGTGTCATCCCAAGAGTCTC |
Tgfb | CTGCTGACCCCCACTGATAC | AGCCCTGTATTCCGTCTCCT |
cTnI | AAAAAGTCTAAGATCTCCGCCTCCA | GGTTTTCCTTCTCAATGTCCTCCTT |
cTnT | CGTAGAAGAGGTTGGTCCTGATGAA | TGTACCCTCCAAA |