Fig. 3
Defining two molecularly distinct populations in the key branch point on day 17. a The branch point between day 11 and 17 during DA differentiation. b C5 and C6 clusters showing expression of DA progenitors-related genes shown as violin plots. Violin plots showing the expression of marker genes of C5 and C6 clusters. c Volcano plot of significantly differentially expressed genes (|log2FC|> 1; padj < 0.05) between C5 and C6 cluster. d Immunofluorescence detection of TH positive after the hESCs cell lines of overexpressing LGI1 differentiation into DA. n.s., not significant. Data are shown as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. Scale bars, 50 μm. e Quantification of the percentage of TH+ cells on day 25 of differentiation. Dox was added on day 7 and day 11, respectively. Data are shown as mean ± SEM, n = 3. **P < 0.01, ***P < 0.001