Fig. 2

Intra-amniotic transplanted BDNF-BMSCs implanted into defective spinal cord and skin. A. Typical images observed under a stereoscopic fluorescence microscope; the white box is magnified, and the scale: 2.5 mm; the yellow dotted line indicates the location of the rightmost frozen section which shows that the transplanted bone marrow mesenchymal stem cells (BMSCs) engrafted into the defective spinal cord and the edge of the broken skin, scale: 100 μm. B: Quantitative analysis of the relative mRNA expression of BDNF in SBA spinal cords of PBS-injected, BMSC-injected and BDNF-BMSC-injected fetuses (n = 12 per group). C: The protein expression of BDNF and GAPDH in the defective spinal cord was detected by the simple western system in PBS-, BMSC-, and BDNF-BMSC-injected groups. D. Quantification of relative protein levels of BDNF in different transplantation groups from Figure C. *Compared to the PBS-injected group, p < 0.05; †Compared to the BMSC-injected group, p < 0.05. E. Representative images of E20 rat fetus with SBA after PBS injection, BMSC transplantation, and BDNF-BMSC transplantation taken by fluorescent stereomicroscopy; the transplanted cells were labeled by GFP. The white dots indicate the edge of the defective skin lesions. F. Quantitative analysis of the area of skin lesions in the BDNF-BMSC-injected, BMSC-injected, and PBS-injected groups (n = 23 per group). *Compare to the PBS-injected group, p < 0.05