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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Biological activity reduction and mitochondrial and lysosomal dysfunction of mesenchymal stem cells aging in vitro

Fig. 2

The biological activity of UMSC-P4 and UMSC-P9. a Representative images of Transwell migration assays of UMSC-P4 and UMSC-P9. Scale bar, 50 µm. b Quantification of migrated cell numbers in UMSC-P4 and UMSC-P9. At least 20 representative visual fields for each group were counted. c UMSCs were differentiated into osteoblasts for 15 days and then analyzed by alizarin red staining. Scale bar, 50 µm. d UMSCs were differentiated into adipocytes for 20 days and then analyzed by oil red-O staining.  Scale bar, 50 µm. e, f Inhibitory effects of UMSCs on the proliferation of CD4+ T cells and CD8+ T cells, e Representative images of flow cytometry of proliferating CD4+ T cells and CD8+ T cells in different groups. Unstimulated T cells have a single peak at a high fluorescent. Upon stimulated, lower fluorescent intensity peaks form. f Quantification of the relative percentage of proliferating CD4+ T cells and CD8+ T cells in different groups (n ≥ 3). The relative percentages of CD4+ T cells and CD8+T cells in control group were set as 100%. ***P < 0.001 compared to control

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