Fig. 4

Decreased response to oxidative stress and increased ferroptosis of the mitochondria in UMSC-P9. a Representative flow cytometry image of ROS levels detected by H2-DCFDA and Quantification of ROS levels in UMSC-P4 and UMSC-P9 (n ≥ 3). b Quantification of apoptosis percentages of UMSC-P4 and UMSC-P9 treated with H₂O₂ (n ≥ 3). c Representative flow cytometry image of ROS levels and quantification of ROS levels in UMSC-P4 and UMSC-P9 treated with H₂O₂ (n ≥ 3). d Representative mitochondrial images of UMSC-P4 and UMSC-P9 treated with H₂O₂ stained with MitoTracker Red. Scale bar, 50 µm. e Representation of TEM images of the mitochondria of UMSC-P4 and UMSC-P9. The red arrow indicates the mitochondrion with the reduction and disappearance of mitochondrial cristae. f Representative flow cytometry images of lipid ROS levels and quantification of lipid ROS levels in UMSC-P4 and UMSC-P9 (n ≥ 3). g Total GSH levels in the cell lysate in UMSC-P4 and UMSC-P9 (n ≥ 3). The concentration of UMSC-P4 was set as 1. h Western Blot analysis of GPX4 levels in UMSC-P4 and UMSC-P9. i Representative mitochondrial images of UMSC-P4 and UMSC-P9 stained with MitoTracker Red after treated with 5 µM RSL3 for 24 h. Scale bar, 50 µm. j Representative flow cytometry images of lipid ROS levels and quantification of lipid ROS levels in UMSC-P4 and UMSC-P9 after treated with 2 µM curcumin for 48 h (n ≥ 3). k GPX4 levels were detected in UMSC-P4 and UMSC-P9 by western blot. l Total GSH levels in the cell lysate in UMSC-P4 and UMSC-P9 (n ≥ 3). The concentration of UMSC-P4 and UMSC-P4 treated with DMSO was set as 1, respectively. m Representative mitochondrial images of UMSC-P4 and UMSC-P9 stained with MitoTracker Red after treated with curcumin. Scale bar, 50 µm. White arrows represent punctate fragments of mitochondria