Table 1 Gene-specific primers used in the real-time quantitative PCR
Gene | Primer sequences (5′-3′) |
|---|---|
Beclin-1 | Forward 5′-TCATGCGATGGTGGCTTTCC-3′ Reverse 5′-ATGGAATAGGAGCCGCCACT-3′ |
ATG5 | Forward 5′-ACCTTTGCAGTGGCTGAGTG-3′ Reverse 5′-TCAATCTGTTGGTTGCGGGA-3′ |
ATG12 | Forward 5′-CAACTGCTGCTGAGGGCGATG-3′ Reverse 5′-CACCGGCAGGTTCTTCTGTTCC-3′ |
P62 | Forward 5′-CTGATGAAGGTGGCTGGCTGAC-3′Reverse 5′-CAAGGGCGGTGGGTGTTTCG-3′ |
PI3K | Forward 5′-ACGGAGGAGGTGCTCTGGAAC-3′ Reverse 5′-GGACTCGGGACTGGGCATCTC-3′ |
Akt | Forward 5′-GACGGCACCTTCATCGGCTAC-3′ Reverse 5′-CGCCACGGAGAAGTTGTTGAGG-3′ |
mTOR | Forward 5′-GCACGTCAGCACCATCAACCTC-3′ Reverse 5′-GCCTCAGCCATTCCAACCAGTC-3′ |