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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Diabetic microenvironment preconditioning of adipose tissue-derived mesenchymal stem cells enhances their anti-diabetic, anti-long-term complications, and anti-inflammatory effects in type 2 diabetic rats

Fig. 1

Identification of ADSCs under diabetic microenvironment and the cell secretion effect of ADSCs was enhanced. Immune-phenotype of diabetic microenvironment pre-MSCs by flow cytometry (A). Representative photomicrographs of ADSCs and diabetic microenvironment pre-ADSCs and displayed similar spindle shaped morphology (B). Annexin V-PE/7-AAD cell apoptosis detected cell necrosis and late apoptosis, and MTT assay is used to detect cell proliferation activity (C–D). Take different concentrations, stimulate different time, qRT-PCR results of cytokines (E). Secreted cytokine of cell supernatant by ELISA test (F). Data are presented as the mean ± SEM of three separate experiments. l-precondition: low concentration precondition. h-precondition: high concentration precondition

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Stem Cell Research & Therapy

ISSN: 1757-6512