Fig. 1

MTT assay for assessment of the impact of PrPc^OE on cell viability and cell cycle. A Cell viability at 24 h, * versus other groups with different symbols (†, ‡), p < 0.0001. B Cell viability at 48 h, * versus other groups with different symbols (†, ‡), p < 0.0001. C Cell viability at 72 h, * versus other groups with different symbols (†, ‡), p < 0.0001. D–F) Illustrating the flow cytometric analysis for determining the cell cycle activity in EPCs only D, EPCs + H₂O₂ E and H₂O₂ + PrPc^OE-EPCs F, respectively. G Analytical result of the expression of Sub-G1 phase of cell cycle, * versus other groups with different symbols (†, ‡), p < 0.0001. H Analytical result of the expression of G0/G1 phase of cell cycle, * versus other groups with different symbols (†, ‡), p < 0.0001. I Analytical result of the expression of S phase of cell cycle, * versus other groups with different symbols (†, ‡), p < 0.0001. J Analytical result of the expression of G2/M phase of cell cycle, * versus †, p < 0.01. All statistical analyses were performed by one-way ANOVA, followed by Bonferroni multiple comparison post hoc test (n = 8 for each group). Data were expressed as mean ± SE. Symbols (*, †, ‡) indicate significance (at 0.05 level). EPCs = endothelial progenitor cells; H₂O₂ = hydrogen peroxide; and PrPc^OE-EPCs = overexpression of cellular prion protein in EPCs