Fig. 3From: Rejuvenated endothelial progenitor cells through overexpression of cellular prion protein effectively salvaged the critical limb ischemia in rats with preexisting chronic kidney diseaseImpact of PrPcOE on cell migratory and angiogenesis capacity. A–F illustrating the microscopic finding for identification of cell migratory capacity (i.e., by Migratory assay). A–C: indicated 100 × under microscopic finding; scale bars in right lower corner represent 100 µm. D to F: indicated 200 × under microscopic finding; scale bars in right lower corner represent 50 µm. G Analytical result of number of cell migration, * versus other group with different symbols (†, ‡, §), p < 0.0001. H–J Illustrating the morphological features (200×) of Matrigel assay for identification of angiogenesis in EPCs, PrPcOE-EPCs and siPrnp-EPCs, respectively. The parameters of angiogenesis, including: (1) tubular formation (red arrows), (2) cluster formation (yellow arrows) and (3) network formation (green color). K Analytical result of number of tubules, * versus other groups with different symbols (†, ‡), p < 0.0001. L Analytical result of total tubular length, * versus other groups with different symbols (†, ‡), p < 0.0001. M Analytical result of mean tubular length, * versus other groups with different symbols (†, ‡), p < 0.0001. N Analytical result of cluster formation, * versus other groups with different symbols (†, ‡), p < 0.0001. O Analytical result of network formation, * versus other groups with different symbols (†, ‡), p < 0.0001. Scale bar in right lower corner represents 50 µm. All statistical analyses were performed by one-way ANOVA, followed by Bonferroni multiple comparison post hoc test (n = 8 for each group). Symbols (*, †, ‡) indicate significance (at 0.05 level). EPCs = endothelial progenitor cells; PrPcOE-EPCs = overexpression of cellular prion protein in EPCs. siPrnp-EPCs = knockdown of cellular prion protein in EPCsBack to article page