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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Rejuvenated endothelial progenitor cells through overexpression of cellular prion protein effectively salvaged the critical limb ischemia in rats with preexisting chronic kidney disease

Fig. 3

Impact of PrPcOE on cell migratory and angiogenesis capacity. AF illustrating the microscopic finding for identification of cell migratory capacity (i.e., by Migratory assay). AC: indicated 100 × under microscopic finding; scale bars in right lower corner represent 100 µm. D to F: indicated 200 × under microscopic finding; scale bars in right lower corner represent 50 µm. G Analytical result of number of cell migration, * versus other group with different symbols (†, ‡, §), p < 0.0001. HJ Illustrating the morphological features (200×) of Matrigel assay for identification of angiogenesis in EPCs, PrPcOE-EPCs and siPrnp-EPCs, respectively. The parameters of angiogenesis, including: (1) tubular formation (red arrows), (2) cluster formation (yellow arrows) and (3) network formation (green color). K Analytical result of number of tubules, * versus other groups with different symbols (†, ‡), p < 0.0001. L Analytical result of total tubular length, * versus other groups with different symbols (†, ‡), p < 0.0001. M Analytical result of mean tubular length, * versus other groups with different symbols (†, ‡), p < 0.0001. N Analytical result of cluster formation, * versus other groups with different symbols (†, ‡), p < 0.0001. O Analytical result of network formation, * versus other groups with different symbols (†, ‡), p < 0.0001. Scale bar in right lower corner represents 50 µm. All statistical analyses were performed by one-way ANOVA, followed by Bonferroni multiple comparison post hoc test (n = 8 for each group). Symbols (*, †, ‡) indicate significance (at 0.05 level). EPCs = endothelial progenitor cells; PrPcOE-EPCs = overexpression of cellular prion protein in EPCs. siPrnp-EPCs = knockdown of cellular prion protein in EPCs

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